WebThe SLC35C1 knockout HEK293T and HepG2 cells were transfected with the obtained vector using the FuGENE HD transfection reagent according to the manufacturer’s ... of involvement of UGT1 and UGT2 splice variants of UDP-galactose transporter in glycosylation of macromolecules in MDCK and CHO cell lines. Glycoconjugate J. 2011; … Web2 dagen geleden · (H) MDCK cells expressing Booster-PKA in the presence of the inhibitors were imaged and analyzed for the RSPA frequency. Reagents are as follows: DMSO, 0.1% v/v DMSO; PKAi, 20 µM H89; Apyrase, 10 unit mL -1; EP2i, 10 µM PF-04418948; EP4i, 1 µM ONO-AE3-208; COXi, 10 µM indomethacin. The frequency of RSPA was analyzed …

4D-Nucleofector™ Protocol for MDCK Cells - Lonza

Web2 apr. 2013 · siRNA at a final concentration of 50 nM were transfected by Lullaby, siRNA transfection reagent in MDCK cell line. this article demonstrate the high efficiency of … WebCat # SL100489-MDCK Store at 4 0 C GenJet In Vitro DNA Transfection Reagent for MDCK Cells (Ver. II)-----A Protocol for Transfecting MDCK Cells 100 µl 500 µl 1000 µl … unknown cars

GeneJuice® Transfection Reagent 70967

Web69. Gently add the transfection solution dropwise onto the cells seeded in 24-well plates in Step 66 and mix by tilting the plate. Allow the cells to continue to grow for approximately 48-hours. 70. Nuclease-containing lysate preparation. Approximately 48-hours post-transfection, prepare fresh lysis buffer as described in the Materials section. 71. Web25 mei 2016 · Apobec-1 complementation factor (A1CF) is a heterogeneous nuclear ribonuceloprotein (hnRNP) and mediates apolipoprotein-B mRNA editing. A1CF can promote the regeneration of the liver by post-transcriptionally stabilizing Interleukin-6 (IL-6) mRNA. It also contains two transcriptional variants-A1CF64 and A1CF65, distinguished … Web26 mrt. 2013 · For MDCK cells, the cells were seeded on 96-well plate and three target-specific siRNAs (Sigma-Aldrich, Inc., St. Louis, MO) or non-targeting negative control … unknown cd