WebMar 22, 2012 · Computational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, encodes the putative endolysin.ORF43, which is 429 bp long and upstream of the lySMP gene, is predicted to encode a putative holin protein, designated HolSMP (Figure … WebEndolysin is the term for muralytic enzymes that degrade the cell wall; endolysins accumulate in the cytosol fully folded during the vegetative cycle. ... Holins constitute one of the most diverse functional groups, with >100 known or putative holin sequences, which form >30 ortholog groups. Publication types Research Support, Non-U ...
Endolysin - an overview ScienceDirect Topics
WebApr 11, 2024 · An ORF 161 consisting of 137 amino acids was annotated as a putative endolysin (GenBank accession QYC50633), namely LysJNwz. Conserved Domain analysis further revealed LysJNwz belongs to the L-Ala-D-Glu peptidase-like family of peptidase_ M15 superfamily with a single conserved domain between amino acids 9–136. WebJul 21, 2024 · In this study, an open reading frame encoding putative endolysin was cloned from the complete genome data and expressed in the Escherichia coli expression system. … tailgate hammock holder
A Thermostable Salmonella Phage Endolysin, Lys68, with Broad
WebMar 10, 2016 · The PAC4 phage genome also has a 94 bp insert located at 15,793 bp in the putative endolysin encoding orf. This creates a stop codon, and divides this sequence into two orfs. While the product of one orf retains the region encoding an intact amidase domain, and is 98% homologous to a putative endolysin from P. WebMar 1, 2024 · Clostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. … WebAug 1, 2011 · The putative endolysin gene (ORF24) previously determined in the phage BFK20 genome (EMBL accession no. AJ278322) had to be corrected from 576 bp (ORF24) to 813 bp (ORF24′) because sequencing errors were detected which resulted in a frameshift mutation. The endolysin of BFK20 (gp24′) contains 270 aa, which corresponds to a 30.1 … twilight27